A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis.
Identifieur interne : 000197 ( Main/Exploration ); précédent : 000196; suivant : 000198A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis.
Auteurs : Flavia Q. Pirih ; Sarah Hiyari ; Ho-Yin Leung ; Ana D V. Barroso [Brésil] ; Adrian C A. Jorge [Brésil] ; Jeniffer Perussolo [Brésil] ; Elisa Atti ; Yi-Ling Lin ; Sotirios Tetradis ; Paulo M. CamargoSource :
- The Journal of oral implantology [ 1548-1336 ] ; 2015.
Abstract
Dental implants are a widely used treatment option for tooth replacement. However, they are susceptible to inflammatory diseases such as peri-implant mucositis and peri-implantitis, which are highly prevalent and may lead to implant loss. Unfortunately, the understanding of the pathogenesis of peri-implant mucositis and peri-implantitis is fragmented and incomplete. Therefore, the availability of a reproducible animal model to study these inflammatory diseases would facilitate the dissection of their pathogenic mechanisms. The objective of this study is to propose a murine model of experimental peri-implant mucositis and peri-implantitis. Screw-shaped titanium implants were placed in the upper healed edentulous alveolar ridges of C57BL/6J mice 8 weeks after tooth extraction. Following 4 weeks of osseointegration, Porphyromonas gingivalis -lipolysaccharide (LPS) injections were delivered to the peri-implant soft tissues for 6 weeks. No-injections and vehicle injections were utilized as controls. Peri-implant mucositis and peri-implantitis were assessed clinically, radiographically (microcomputerized tomograph [CT]), and histologically following LPS-treatment. LPS-injections resulted in a significant increase in soft tissue edema around the head of the implants as compared to the control groups. Micro-CT analysis revealed significantly greater bone loss in the LPS-treated implants. Histological analysis of the specimens demonstrated that the LPS-group had increased soft tissue vascularity, which harbored a dense mixed inflammatory cell infiltrate, and the bone exhibited noticeable osteoclast activity. The induction of peri-implant mucositis and peri-implantitis in mice via localized delivery of bacterial LPS has been demonstrated. We anticipate that this model will contribute to the development of more effective preventive and therapeutic approaches for these 2 conditions.
DOI: 10.1563/aaid-joi-D-14-00068
PubMed: 24967609
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000C63
- to stream PubMed, to step Curation: 000C63
- to stream PubMed, to step Checkpoint: 000C63
- to stream Ncbi, to step Merge: 000E89
- to stream Ncbi, to step Curation: 000E89
- to stream Ncbi, to step Checkpoint: 000E89
- to stream Main, to step Merge: 000197
- to stream Main, to step Curation: 000197
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis.</title>
<author><name sortKey="Pirih, Flavia Q" sort="Pirih, Flavia Q" uniqKey="Pirih F" first="Flavia Q" last="Pirih">Flavia Q. Pirih</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Hiyari, Sarah" sort="Hiyari, Sarah" uniqKey="Hiyari S" first="Sarah" last="Hiyari">Sarah Hiyari</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Leung, Ho Yin" sort="Leung, Ho Yin" uniqKey="Leung H" first="Ho-Yin" last="Leung">Ho-Yin Leung</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Barroso, Ana D V" sort="Barroso, Ana D V" uniqKey="Barroso A" first="Ana D V" last="Barroso">Ana D V. Barroso</name>
<affiliation wicri:level="1"><nlm:affiliation>2 Universidade Federal do Espirito Santo, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>2 Universidade Federal do Espirito Santo, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jorge, Adrian C A" sort="Jorge, Adrian C A" uniqKey="Jorge A" first="Adrian C A" last="Jorge">Adrian C A. Jorge</name>
<affiliation wicri:level="1"><nlm:affiliation>3 Universidade Estadual de Ponta Grossa, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>3 Universidade Estadual de Ponta Grossa, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Perussolo, Jeniffer" sort="Perussolo, Jeniffer" uniqKey="Perussolo J" first="Jeniffer" last="Perussolo">Jeniffer Perussolo</name>
<affiliation wicri:level="1"><nlm:affiliation>3 Universidade Estadual de Ponta Grossa, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>3 Universidade Estadual de Ponta Grossa, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Atti, Elisa" sort="Atti, Elisa" uniqKey="Atti E" first="Elisa" last="Atti">Elisa Atti</name>
<affiliation><nlm:affiliation>4 University of California, Los Angeles, School of Dentistry, Section of Oral Radiology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Lin, Yi Ling" sort="Lin, Yi Ling" uniqKey="Lin Y" first="Yi-Ling" last="Lin">Yi-Ling Lin</name>
<affiliation><nlm:affiliation>5 University of California, Los Angeles, School of Dentistry, Section of Oral Pathology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Tetradis, Sotirios" sort="Tetradis, Sotirios" uniqKey="Tetradis S" first="Sotirios" last="Tetradis">Sotirios Tetradis</name>
<affiliation><nlm:affiliation>4 University of California, Los Angeles, School of Dentistry, Section of Oral Radiology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Camargo, Paulo M" sort="Camargo, Paulo M" uniqKey="Camargo P" first="Paulo M" last="Camargo">Paulo M. Camargo</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:24967609</idno>
<idno type="pmid">24967609</idno>
<idno type="doi">10.1563/aaid-joi-D-14-00068</idno>
<idno type="wicri:Area/PubMed/Corpus">000C63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C63</idno>
<idno type="wicri:Area/PubMed/Curation">000C63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000C63</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000C63</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000C63</idno>
<idno type="wicri:Area/Ncbi/Merge">000E89</idno>
<idno type="wicri:Area/Ncbi/Curation">000E89</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000E89</idno>
<idno type="wicri:Area/Main/Merge">000197</idno>
<idno type="wicri:Area/Main/Curation">000197</idno>
<idno type="wicri:Area/Main/Exploration">000197</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis.</title>
<author><name sortKey="Pirih, Flavia Q" sort="Pirih, Flavia Q" uniqKey="Pirih F" first="Flavia Q" last="Pirih">Flavia Q. Pirih</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Hiyari, Sarah" sort="Hiyari, Sarah" uniqKey="Hiyari S" first="Sarah" last="Hiyari">Sarah Hiyari</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Leung, Ho Yin" sort="Leung, Ho Yin" uniqKey="Leung H" first="Ho-Yin" last="Leung">Ho-Yin Leung</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Barroso, Ana D V" sort="Barroso, Ana D V" uniqKey="Barroso A" first="Ana D V" last="Barroso">Ana D V. Barroso</name>
<affiliation wicri:level="1"><nlm:affiliation>2 Universidade Federal do Espirito Santo, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>2 Universidade Federal do Espirito Santo, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jorge, Adrian C A" sort="Jorge, Adrian C A" uniqKey="Jorge A" first="Adrian C A" last="Jorge">Adrian C A. Jorge</name>
<affiliation wicri:level="1"><nlm:affiliation>3 Universidade Estadual de Ponta Grossa, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>3 Universidade Estadual de Ponta Grossa, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Perussolo, Jeniffer" sort="Perussolo, Jeniffer" uniqKey="Perussolo J" first="Jeniffer" last="Perussolo">Jeniffer Perussolo</name>
<affiliation wicri:level="1"><nlm:affiliation>3 Universidade Estadual de Ponta Grossa, School of Dentistry, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>3 Universidade Estadual de Ponta Grossa, School of Dentistry</wicri:regionArea>
<wicri:noRegion>School of Dentistry</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Atti, Elisa" sort="Atti, Elisa" uniqKey="Atti E" first="Elisa" last="Atti">Elisa Atti</name>
<affiliation><nlm:affiliation>4 University of California, Los Angeles, School of Dentistry, Section of Oral Radiology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Lin, Yi Ling" sort="Lin, Yi Ling" uniqKey="Lin Y" first="Yi-Ling" last="Lin">Yi-Ling Lin</name>
<affiliation><nlm:affiliation>5 University of California, Los Angeles, School of Dentistry, Section of Oral Pathology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Tetradis, Sotirios" sort="Tetradis, Sotirios" uniqKey="Tetradis S" first="Sotirios" last="Tetradis">Sotirios Tetradis</name>
<affiliation><nlm:affiliation>4 University of California, Los Angeles, School of Dentistry, Section of Oral Radiology, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Camargo, Paulo M" sort="Camargo, Paulo M" uniqKey="Camargo P" first="Paulo M" last="Camargo">Paulo M. Camargo</name>
<affiliation><nlm:affiliation>1 University of California, Los Angeles, School of Dentistry, Section of Periodontics, Los Angeles, Calif.</nlm:affiliation>
<wicri:noCountry code="subField">Calif</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of oral implantology</title>
<idno type="eISSN">1548-1336</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Dental implants are a widely used treatment option for tooth replacement. However, they are susceptible to inflammatory diseases such as peri-implant mucositis and peri-implantitis, which are highly prevalent and may lead to implant loss. Unfortunately, the understanding of the pathogenesis of peri-implant mucositis and peri-implantitis is fragmented and incomplete. Therefore, the availability of a reproducible animal model to study these inflammatory diseases would facilitate the dissection of their pathogenic mechanisms. The objective of this study is to propose a murine model of experimental peri-implant mucositis and peri-implantitis. Screw-shaped titanium implants were placed in the upper healed edentulous alveolar ridges of C57BL/6J mice 8 weeks after tooth extraction. Following 4 weeks of osseointegration, Porphyromonas gingivalis -lipolysaccharide (LPS) injections were delivered to the peri-implant soft tissues for 6 weeks. No-injections and vehicle injections were utilized as controls. Peri-implant mucositis and peri-implantitis were assessed clinically, radiographically (microcomputerized tomograph [CT]), and histologically following LPS-treatment. LPS-injections resulted in a significant increase in soft tissue edema around the head of the implants as compared to the control groups. Micro-CT analysis revealed significantly greater bone loss in the LPS-treated implants. Histological analysis of the specimens demonstrated that the LPS-group had increased soft tissue vascularity, which harbored a dense mixed inflammatory cell infiltrate, and the bone exhibited noticeable osteoclast activity. The induction of peri-implant mucositis and peri-implantitis in mice via localized delivery of bacterial LPS has been demonstrated. We anticipate that this model will contribute to the development of more effective preventive and therapeutic approaches for these 2 conditions.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
</country>
</list>
<tree><noCountry><name sortKey="Atti, Elisa" sort="Atti, Elisa" uniqKey="Atti E" first="Elisa" last="Atti">Elisa Atti</name>
<name sortKey="Camargo, Paulo M" sort="Camargo, Paulo M" uniqKey="Camargo P" first="Paulo M" last="Camargo">Paulo M. Camargo</name>
<name sortKey="Hiyari, Sarah" sort="Hiyari, Sarah" uniqKey="Hiyari S" first="Sarah" last="Hiyari">Sarah Hiyari</name>
<name sortKey="Leung, Ho Yin" sort="Leung, Ho Yin" uniqKey="Leung H" first="Ho-Yin" last="Leung">Ho-Yin Leung</name>
<name sortKey="Lin, Yi Ling" sort="Lin, Yi Ling" uniqKey="Lin Y" first="Yi-Ling" last="Lin">Yi-Ling Lin</name>
<name sortKey="Pirih, Flavia Q" sort="Pirih, Flavia Q" uniqKey="Pirih F" first="Flavia Q" last="Pirih">Flavia Q. Pirih</name>
<name sortKey="Tetradis, Sotirios" sort="Tetradis, Sotirios" uniqKey="Tetradis S" first="Sotirios" last="Tetradis">Sotirios Tetradis</name>
</noCountry>
<country name="Brésil"><noRegion><name sortKey="Barroso, Ana D V" sort="Barroso, Ana D V" uniqKey="Barroso A" first="Ana D V" last="Barroso">Ana D V. Barroso</name>
</noRegion>
<name sortKey="Jorge, Adrian C A" sort="Jorge, Adrian C A" uniqKey="Jorge A" first="Adrian C A" last="Jorge">Adrian C A. Jorge</name>
<name sortKey="Perussolo, Jeniffer" sort="Perussolo, Jeniffer" uniqKey="Perussolo J" first="Jeniffer" last="Perussolo">Jeniffer Perussolo</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000197 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000197 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:24967609 |texte= A Murine Model of Lipopolysaccharide-Induced Peri-Implant Mucositis and Peri-Implantitis. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24967609" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a EdenteV1
This area was generated with Dilib version V0.6.33. |